Icb-pharma.ums.ac.id
INTERNATIONAL CONFERENCEICB PHARMA II
PROGRAMME
ABSTRACT BOOK
"Current Breakthrough in
Pharmacy Materials and Analyses"
Faculty of Pharmacy
Universitas Muhammadiyah Surakarta
Pharmacy Faculty, Universitas Muhammadiyah Surakarta Surakarta, Indonesia Chairman of Editors : Erindyah Retno Wikantyasning, Ph.D., Apt. Tanti Azizah Sujono, M.Sc., Apt. Team of Pharmaceutical Technology : Anita Sukmawati, Ph.D., Apt. Suprapto, M.Sc.,Apt. Erindyah Retno Wikantyasning, Ph.D., Apt. Gunawan Setiyadi, M.Sc., Apt. Team of Pharmacology and Microbiology : Azis, Saifudin, Ph.D., Apt. Arifah Sri Wahyuni, M.Sc., Apt. Ratna Yuliani, M.Biotech.St. Tanti Azizah Sujono, M.Sc., Apt. Team of Clinical and Community Pharmacy : Dra. Nurul Mutma'inah, M.Si., Apt. Zakky Cholisoh, M.Clin. Pharm., Ph.D., Apt. Hidayah Karuniawati, M.Sc. Apt. Team of Pharmaceutical Chemistry : Dedi Hanwar, M.Si., Apt. Dr. Muhammad Da'i, M.Si., Apt. Dr. Muhtadi Ibrahim, M.Sc. Broto Santoso, M.Sc., Apt. Andi Suhendi, M.Sc., Apt. Ika Trisharyanti, M.Sc., Apt. Team of Molecular Biology : Agus Purnomohadi, M.Sc. Maryati, Ph.D., Apt. Copyright 2015
Copyright in compilers and reserved Design cover: Publication and Documentation Team Layout: Secretary and IT Team Published by:
Muhammadiyah University Press
Universitas Muhammadiyah Surakarta Jl. A. Yani Pabelan Tromol Pos I Kartasura Surakarta 57102 Telp. (+62 271) 717417-172, E-mail: [email protected] ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" It's my great pleasure to welcome you to the 2nd International Current Breakthrough (ICB)-Pharma Symposium 2015 in Solo Indonesia which will be held on 31 October, 2015 under the auspices of Universitas Muhammadiyah Surakarta. ICB-Pharma 2015 will feature a theme of "Current Breakthrough in Pharmacy Material and Analyses" and will consist of morning and afternoon sessions. There will be plenary lectures and session lectures given by several invited speakers from Singapore, Japan, as well as from Indonesia, and also selected oral presentations of the submitted papers. The poster session from various fields of pharmaceutical sciences will take place nearby. The ICB-Pharma will be directed for a tradition and in the future will be nurtured as a well-known scientific symposium in disseminating the breakthrough and novel technology in pharmacy materials. This purpose will be able to achieve by encouragement of national and international academic institution partners and supports from the industrial partners, Indonesian Pharmacist Association (Ikatan Apoteker Indonesia, IAI), colleagues and from the organizing committee. Moreover, I do hope and believe that, the 2nd ICB-Pharma will offer great opportunities for the scientists to meet and discuss recent topics in the field of material and pharmaceutical science and bring the academics, health professionals and industries together for sharing their experiences to solve current problems and challenges in practice. Azis Saifudin, PhD, Apt.
Chair of ICB-Pharma Symposium
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" This conference is held to disseminate current methods which provide advanced materials and methods in pharmacy. This is a good media for those who are engaged in academic, industrial, regulatory fields to conduct social interactions, to share their findings, to communicate bottle neck surrogates, and to seek the possibilities for collaborations. We are quite humble to recognize our weakness in running all agendas. Hence, from bottom of our heart we ask apologizes from all participants for any service, lack facilities, low response, etc. However, we must be persistent and ensuring our positive contribution toward scientific society especially at the attempt to develop capacity building of pharmaceutical sciences in Indonesia. Thus, we will run ICB Pharmacy To all participants, I wish an inspiring moment in Solo city, a city where was born a national leader and city of heritage! Azis Saifudin, PhD, Apt.
Dean of Faculty of Pharmacy
Universitas Muhammadiyah Surakarta
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" TABLE OF CONTENTS
PREFACE . iii
TABLE OF CONTENTS . v
PROGRAMME . ix
ORAL PRESENTER ROOM. x
LIST OF PARTICIPANT NON PRESENTERS . xi
LIST OF ORAL PRESENTERS . xvi
LIST OF POSTER PRESENTERS . xviii
SPEAKER . 1
A-PHARMACEUTICAL TECHNOLOGY . 4
A001
OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" B-PHARMACOLOGY AND MICROBIOLOGY . 12
B001
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITH WRITHING REFLEX METHOD C- CLINICAL AND COMMUNITY PHARMACY . 26
C001
D-PHARMACEUTICAL CHEMISTRY . 31
D001
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" E-MOLECULAR BIOLOGY . 34
E001
CV OF SPEAKER . 37 LIST OF COMMITTEES . 40 MAP OF FACULTY OF PHARMACY . 42 LIST OF TAXI AND HOTELS . 43 ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PROGRAMME
Venue:
Auditorium Moh. Djazman, Universitas Muhammadiyah Surakarta
Jl. Ahmad Yani, Tromol Pos I, Pabelan, Surakarta 57162 Indonesia
Session:
Morning Session
07.00-08.00
Registration for participant Opening Ceremony Morning Coffee Break Speaker 1 Prof. Jackie Ying (Executive Director, Institute of Bio-engineering and Nanotechnology) Topic: "Current status on nano medicine and nano devices" Speaker 2 Assoc. Prof. Takenori Tomohiro (University of Toyama, Japan). Topic: "Photoaffinity labeling-based target identification of bioactive molecules" Speaker 3 Dr. Wangsa Tirta Ismaya (Scientist/Head section of Recombinant therapeutic proteins, PT. Dexa Medica). Topic: "Current status of recombinant therapeutic proteins and local issues related" Afternoon Session
13.00-13.30
Oral Presentation Session Closing Ceremony ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" ORAL PRESENTER ROOM
Presenter
Moderator
Gunawan Setiyadi, M.Sc., Andi Suhendi, MSc., Apt. INDRI KUSUMA DEWI Dedi Hanwar, M.Si., Apt. Maryati, Ph.D., Apt. RETNO ARIANINGRUM ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" LIST OF PARTICIPANT NON PRESENTERS
Institution
HIMYATUL HIDAYAH PRODI MAGISTER FARMASI UMS PRODI MAGISTER FARMASI UMS DITA MARINA LUPITANINGRUM PRODI MAGISTER FARMASI UMS UMI KHOLIFAH.S.FARM.,APT APOTEK KARUNIA FARMA LENI HERLINA WIDIASARI, S.SI.,APT. APOTEK WALI SEHAT SEMARANG DHIGNA LUTHFIYANI CITRA UNIVERSITAS JAMBI YUSNITA APRILIYANA APOTEK SIDAPURNA NOVI DIANASARI, S.FARM, APT. APOTIK KONDANG WARAS DEVI UTAMI YULISTYANTI S. FARM., ELLA SKIN CARE SOLO DIKA HERNAWATI S.FARM., APT ELLA SKIN CARE SOLO KLINIK INSANI MEDICA SUSILOWATI.,S.SI.,APT DRA. DWI MURTINGHASTUTI,APT AGNES RINA SRI MURWANI, DWI KARTIKA SANTI RS PKU MUHAMMADIYA KARTASURA SRI HARTINI, S.FARM.APT APOTEK SUMBER SEHAT BOYOLALI NUR SEKTI HIDAYAT RAHMAWATI S. FARM., APT. DIAZ VEGA AKHIRUNNISA, S.FARM., ANNISA RIZKI MURDIYANI PT. DAYA MUDA AGUNG UMI KHOLIFAH.S.FARM.,APT APOTEK KARUNIA FARMA HANDIKA DESI YANOTAMA SMK ASSHODIQIYAH SEMARANG RETNO HARI WAHYUNI CAESNAN MARENDRA GRAHAN UNIVERSITAS KRISTEN IMMANUEL SARAH PUSPITA ATMAJA AIRLANGGA UNIVERSITY APOTEK HARJOSARI FARMA APOTEK DAWA FARMA INAYATUL HIDAYATI ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" Institution
MARLIN TIGOR S.SI. APT LENI HERLINA WIDIASARI SSI.,APT APOTIK WALI SEHAT ARRYSKA AYU PERMATASARI LINNA MARLINNA S.SI, APT IAI PC KAB. TEGAL HENDRA ANTAN DJAYA, S.SI, MBA, IAI PC KAB. TEGAL QUROTUL AINI, S.FARM.,APT APOTEK MITRA MEDIKA STIKES BAKTI TUNAS HUSADA FRANSISCA MEINDRIA NARASTY, PT. GRAHA FARMA SURAKARTA DESTANTIA NADYA AMANTHA STIFAR YAYASAN PHARMASI SEMARANG STIFAR YAYASAN PHARMASI TYAS SARININGRUM S.FARM., APT CANDRA EKA PUSPITASARI UNIVERSITAS GADJAH MADA DHIGNA LUTHFIYANI CITRA UNIVERSITAS JAMBI FARIDHA CYNTHIA DHEWANTI TRI YOGO WIBOWO UMS AHMAD RIFQI KURNIA UMS CHOIRUL MA'ARIF UMS RAFA ENBUN RELIGIA UMS SOVY SAPTA NUARI PRAMOLIS NAUFAL SATRIA HUTOMO HESTU PUTRI MITAYANI SANGGITA AYU IKASARI UMS DYAH RISWARI PITALOKA ALIFAH ANASTYA DINI UMS RANI UTAMI WIDYANINGRUM MARHAMAH NUR AZIZAH DRAJAT TRI WAHYUDI UMS LITA RAHIMA OENSJAR UMS EKHWAN TRIS WANTO ANDHIKA RIZKY GILANG ULITYATINING TIASARI UMS ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" Institution
AMALIYAH DINA ANGGRAENI AGUNG COKRO PRABOWO ESTUNINGTYAS AYU HAPSARI RISQIE CAHYANING KUSUMASARI IRHAMADI MALIK UMS UMUL BAROROTUY SYAMS UMI NURHAYATI UMS RIZKI OCTAVIANA UMS BAIQ SUPRAMONIKA UMS HAZRINI TANJUNG SARI UMS RENY WIDYA ESTUTI UMS ABULKHAIR ABDULLAH NURYATI KUMAN UMS FITRIANISA FATHUROHMAH IMROATUL CHASANAH UMS MARWIANI ARUM SARI UMS RAHAYU DWI KURNIAWATI JANIKA SUJI KUSUMAWARDANI AYU ANGGRAENY UMS EMAMATUL KHUTSIYAH BUNGA INTAN SAVITRI UMS ANNIE RAHMATILLAH UMS ISTIQAMATUSH SHOLIHAH ANGGRIANA ARISTYA UMS ISNAINI NUR HIDAYAH UMS NAHYATU SUFIAH UMS RAUDAH PUTERI ALMINA ADIVA TANTYAS AURORA FADILA KHOIRUNNISA UMS INTAN RIA NURJANAH UMS SUNJAWA ALIF SAPUTRI UMS KURNIA PUNGKY ASMORO NIKEN DWI MULYASARI ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" Institution
AISYAH PUTRIANI UMS FADHILA DIAH SUMINAR SEKAR PUJI UTAMI UMS WITRI DYAH MEILANI UMS NINA NURMITA HABSARI VERANTIKA DEA INDRASWARI TRI AGUS SAROSO UMS YUDA MARSONO UMS AULIA ANNUR AISYIAH UMS ADHI WARDHANA AMRULLAH FEBRIANNA SURYANINGTYAS ANINDYA SETYOWATI UMS RATNA KARTIKASARI UMS ESTHI UTAMININGSIH UMS YENY DWI NOVITASARI UMS NENI LUGKI NIAN TARY UMS SALLY ASTYA UTAMI UMS TANTIN HAYU SURYA UMS THORIQ MAHMUD UMS RAKIH YUSMA RANGGA HENDRA YUANA UMS GITA AYU PRADINA UMS BERNADI WICAKSONO UMS ROSMA FAUZIAH UMS TESAR ZULMI ANTORO UMS ISTI SETIA HAPSARI UMS BENY DWI HATMOKO UMS ANGGITA SEKAR ARUMSASI DIAN YULISTIA ASTRI UMS DIAN AYU ARA ARA ARTHASARI EKA PRASNAPARAMITA W RIZKA ASTIKAH FITRIANA NUNGKY ASMARANING WAHYONO DEVI AMBARRINI WAHYUNINGTYAS DESTY RIRIN ROHMAWATI EKA PRADITA PUTRI UMS AKHMAD AMINUR RIZKI LAILY IEDA QUNTARI UMS MUHAMMAD PRIYADI UMS ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" Institution
MANGGAR ARUM SHINTYA M. DWI SETIANINGRUM MAKUNTI TITIS MUTALIKAH UMS ORLAN PAKAMBANAN APRILYA SRI RACHMAYANTI UMS AKADEMI FARMASI NASIONAL SALSABIELA DWIYUDRISA SUYUDI LILIS FITRIATUN NISA PRODI MAGISTER FARMASI UMS PRODI MAGISTER FARMASI UMS INES NURFITRIANA PRODI MAGISTER FARMASI UMS PRODI MAGISTER FARMASI UMS KATHLEEN APRIANA KRISTININGRUM JAHAMOU PRODI MAGISTER FARMASI UMS HALIDA SURYADINI PRODI MAGISTER FARMASI UMS CHANDRA EKA PUSPITASARI ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" LIST OF ORAL PRESENTERS
Presenter
Institution
CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA DE COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102 FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION METHOD PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST AGAINST Staphylococcus aureus OF KECOMBRANG (Etlingera elatior (Jack) R.M.Sm.) FLOS EXTRACTS OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND SALAM LEAF WATER FRACTION AS ANTI- THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii (Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus) PRE-CLINICAL STUDY OF CR (III) BASED HYPOGLICEMIC SUPPLEMENT IN –TYPE 2 DIABETIC RATS LISA SOEGIANTO WIDYA SCREENING ANTIBACTERIAL POTENCY OF METABOLITE ENDOPHYTIC FUNGI MANGOSTEEN (Garcinia mangostana L.) ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis) AND BLACK CUMIN (Nigella sativa) AGAINST XANTHINE OXIDASE INHIBITION ON HYPERURICEMIC MICE ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST Escherichia coli AND Shigella sonnei ANTIHYPERCHOLESTEROLEMIC EFFECT OF MURBEI (Morus alba L.) LEAVES AND ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY PROPYLTIOURACIL AND HIGH FAT DIET ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" Presenter
Institution
OVERVIEW ABOUT ANTIBIOTIC'S PRESCRIBING IN URINARY TRACT INFECTION ROEMANI SEMARANG HOSPITAL'S INPATIENTS CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY ON CHILDREN WITH TYPHOID FEVER ANTIOXIDANT ACTIVITY ETHYL ACETATE EXTRACT OF SEED AND POD PARKIA (Parkia speciosa Hassk.) WITH DPPH (1,1 - DIPHENYL - 2 - PICRYLHYDRAZIL) METHOD A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL AZIDES AS A VERSATILE APPROACH TO GENERATE NEW PHARMACOLOGICALLY ACTIVE COMPUNDS CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION ETHANOL EXTRACT OF LEAVES PLANTS SALA (Cynometra ramiflora Linn.) AGAINTS WiDr CELL CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN HeLaA CELLS SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION IN ESCHERICHIA COLI ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" LIST OF POSTER PRESENTERS
Presenter
Institution
ANDI SRI SURIATI PREPARATION OF ARTIFICIAL SALIVA EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY MIXTURE OF PIOGLITAZONE HCl AND METFORMIN HCL THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS ANTIOXIDANT ON THE LOTION MATERIAL ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN (Phyllanthus niruri L.) IN ALLOXAN- INDUCED DIABETIC RATS ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO (Andrographis paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS EXTRACT IN WISTAR RATS THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF WISTAR MALE RATS BLOOD INDUCED BY PARACETAMOL THE PROFILE OF MDA (MALONDIALDEHYDE) LEVEL IN RATS GIVEN EXTRACT OF THYMI HERBS (Thymus vulgaris [L.]) EFFECTIVENESS TEST of 70% ETHANOL EXTRACT ANALGETIK RED DRAGON FRUIT PEEL (Hylocereus polyrhizus Cortex) WITH STRETCHING METHOD ON MALE MICE SWISS WEBSTER STRAIN EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN (Phyllanthus niruri L.) IN ALLOXAN-INDUCED DIABETIC RATS IDA AYU PEBRINA UMS RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA IN ADULT PATIENTS IN THE INSTALLATION INPATIENT HOSPITAL "X" ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" Presenter
Institution
TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND 6 MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROM RIZHOSPHERE OF Zingiber officinale ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" NANOSTRUCTURED BIOMATERIALS FOR MEDICAL AND BIOLOGICAL
Jackie Y. Ying
Institute of Bioengineering and Nanotechnology 31 Biopolis Way, The Nanos, Singapore 138669 E-mail: [email protected]
Nanostructured materials have been developed for various medical and biological applications.
They have been designed as stimuli-responsive drug delivery systems and sustained protein delivery systems. Nanocomposite systems have also been derived to provide simultaneous drug delivery and bioimaging functions as theranostic systems. Micellar nanocomplexes have been synthesized with green tea-based ingredients as unique carrier materials that offer synergistic therapeutic effects with the drugs to be delivered. In addition, nanostructure processing has been employed in creating synthetic cell culture substrates for the expansion and controlled differentiation of stem cells. Nanostructured scaffolds have also been obtained for cell and tissue engineering. ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHOTOAFFINITY LABELING-BASED TARGET IDENTIFICATION OF BIOACTIVE
MOLECULES
Takenori Tomohiro
Graduate School of Medicine and Pharmaceutical Sciences University of Toyama Email: [email protected] Target identification and confirmation for small molecules is often the rate-limiting step in drug discovery. Photoaffinity labeling (PAL) is a photochemical method to attach a tag with a covalent bond into the ligand-interacting surface within target protein. Since PAL can access to most of biological interacting systems including membrane protein or weak-binding protein that are difficult to access by conventional affinity purification methods, it may be a powerful method for identification of target protein that is also able to determine its interacting site. However, conventional PAL-based identification method accompanied with isolation of the target in very pure form often failed, especially in case of the target of a trace amount. The purification process is often complicated by inevitable contamination that is due to non-specific adsorption and significant loss during handling because of the different physical properties of individual peptide fragments. Recently, we designed a unique photoreactive unit that can install a high-performance chemical tag, an isotope-coded fluorescent tag, to the interacting surface upon UV-irradiation. The combination of PAL and heterogeneous target-selecting techniques significantly simplified the procedure and reduced the amount of quantity and time required for identification. The details will be presented. ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" CURRENT STATUS OF RECOMBINANT THERAPEUTIC PROTEINS AND LOCAL
ISSUES RELATED
Wangsa Tirta Ismaya
Recombinant Therapeutic Proteins, Dexa Laboratories of Molecular Sciences, Dexa Medica Industri Selatan V PP-7, Jababeka II Industrial Estate Cikarang 17550, Indonesia E-mail: [email protected] Generation of recombinant version of a therapeutic protein (rTP) has been long employed to solve issues with availability of the protein from its natural resource. Such approach results in production of the protein in large scale of controlled, engineered, and directed manner. However, cost of both generation and production of the biological drug is high, thereby protein theraphy is still not accessible for patients of limited income. Nevertheless, numerous rTPs have been produced in the past decades and their demands keep on increasing. Expiration of patents protecting current rTP allows production of its generic version called biosimilar, which can be made available at lower prices from reduced costs of product development and of pre-clinical and clinical trials. Production of biosimilar is done through processes obtained from technology transfer or self-developed. Either way, validation of the biosimilar product remains the major challenge. Biosimilar product must share identical characteristics and molecular properties, and display similar safety and potency features to those of originator. Alterations in the physico-chemical properties of the molecule or improvement in potency of the drugs are not permitted. On the other hands, development of new recombinant therapeutic protein continues as new diseases or new variant of a disease emerge as well as reoccurrence of well-known diseases. Developing a recombinant therapeutic protein is usually started with search of candidates based on putative or known molecular basis of a disease. We are currently developing a new approach by means of employing proteins of unknown function to screen for their potential use in pharmaceutic or therapy. This approach involves various techniques in bioinformatics, biochemistry, molecular biology and pharmacology, and cell physiology. This approach may lead to discovery of novel pharmaceutical or therapeutic proteins. With the finding of many genes that encode proteins of unknown function during elucidation of genomes (e.g. human), this unusual approach provides alternative to discover new pharmaceutical/therapeutic proteins. We may as well assign possible biological function of the proteins. In Indonesia, development of biosimilar or recombinant therapeutic protein is still at infancy. However, pharmaceutical industries embrace development and production of biosimilar, especially to anticipate the coming Asean Economic Community implementation. The National Agency for Food and Drugs Control (NAFDC) has been developing and preparing guidelines to regulate and control the development and production of both biosimilar and rTP. Related to Indonesian moslem population in particular, development and production of halal certified drugs, including rTP and biosimilar are also part of the major issues. Especially after recently the bill for halal consumer products has been passed. Thus, development of recombinant therapeutic protein appears to gain its momentum in Indonesia in the near future. ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY
CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA DE
COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102
Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya3
1Farmasi, FMIPA, Universitas Sebelas Maret, Surakarta 2Farmasi, FIKES, Universitas Jenderal Soedirman, Purwokerto 3Farmasi, Poltekkes Kemenkes Tasikmalaya, Tasikmalaya *E-mail: [email protected] Abstract
Microcrystalline cellulose is an imported raw material in Indonesia, which used widely as an excipient in tablet production. One of the alternative materials to produce microcrystalline cellulose is from nata de coco. This research aimed to know the characteristic of microcrystalline cellulose from nata de coco compared to avicel pH 101 and avicel pH 102. Nata de coco were alkalinated, dried and hydrolyzed to get microcrystalline cellulose. Independent variables in this research are microcrystalline cellulose from nata de coco, avicel pH 101 and avicel pH 102. While the dependent variables are flow properties, compactibility, compressibility, water absorption, tap density, bulk density, loss of drying, infrared absorption spectra, and SEM images. Data was analyzed using one way ANAVA with CI 95% and using software SPSS for windows. The result showed that the characteristic of microcrystalline cellulose from nata de coco is different in flow properties, compactibility, compressibility, tap density, bulk density, and loss of drying from avicel pH 101 and avicel pH 102, but having the same water absorption. Infrared spectrum data showed that microcrystalline cellulose from nata de coco is similar to avicel pH 101 and avicel pH 102. The SEM result showed that microcrystalline cellulose from nata de coco having bigger particle size (66.67–266.67 μm) than avicel pH 101 (13.33–166.67 μm) and avicel pH 102 (13.33–200 μm). Keywords: Avicel pH 101, Avicel pH 102, Nata de coco, microcrystalline cellulose.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY PREPARATION OF ARTIFICIAL SALIVA FORMULATION
Andi Sri Suriati Amal1*, Samsinah Hj. Hussain2, Mohd. Amin Jalaluddin3
1Universitas Darussalam Gontor, Indonesia 2Universiti Malaya, Malaysia 3Universiti Malaya, Malaysia *E-mail: [email protected] Abstract
Dry mouth or throat (xerostomia) is a clinical condition characterized by desiccation of the intraoral tissues. Patients with chronic or temporary sensation of dry mouth need some kind of treatment to relieve the symptoms. Causes of dry mouth include medications, autoimmune disease (Sjogren's syndrome), radiotherapy or chemotherapy for cancer, hormone disorders and infections. The project is important not only because saliva substitutes are not manufactured locally, but also because most saliva substitutes use mucin (porcin in origin). Therefore there is a need to produce one with other source which has properties to mucin itself. The objective of this project is to produce saliva substitutes that can serve as mouth and throat lubricants. The first step was pre-formulation studies that involved characterization of active ingredients (physical, chemical, and mechanical properties) in order to choose what other ingredients (excipients) should be used in the preparation. Formulation studies also considered such factors as solubility, viscosity, and pH. The last step was assessment of safety and stability of the final product.The new artificial saliva formulations containing various ratios of SCMC (Sodium carboxymethyl cellulose), MC (methyl cellulose) and HPMC (hydroxypropyl methycellulose) have been developed. Combination of cellulose derivatives and albumin in these formulations resulted in the physical properties of these new artificial saliva substitutes closely resembling human saliva and mucin-based saliva substitutes. Formula we choose were the most suitable formulae due to their viscosity and pH properties which closely resemble human saliva and mucin based saliva substitutes.
Keywords: artificial saliva, saliva substitute, mouth and throat lubricant, mouth and throat
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys
zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION METHOD
Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti1
1Department of Pharmacy, University of Sriwijaya Indralaya, OI, South Sumatera, Indonesia *E-mail: [email protected] Abstract
Tunjuk langit rhizome (Helminthostahcys zeylanica (Linn) Hook) has been used traditionally as an anticancer and antiinflamatory agent. In addition, previous studies have proven the potency of ethanol extract from the tunjuk langit rhizome (Helminthostahcys zeylanica (Linn) Hook) as antihyperuricemic agent. In this study, ethanol extract of the rhizome was formulated into a tablet dosage form by a wet granulation method. The tablet was prepared with different types of disintegrant and binder, i.e. Formula A (starch: PVA), Formula B (Avicel®PH102: PVP), and Formula C (sodium alginate: methylcellulose). Physical properties (such as weight variation, tablet diameter, thickness, friability, hardness, and disintegration time) and dissolution of tablets were evaluated. The results showed that tablet A (starch: PVA) produced the best physical properties and dissolution characteristics, which have met the requirements. Therefore, the wet granulation method is suitable to develop the extract into
Keywords: extract, tunjuk langit rhizome, Helminthostachys zaylanica, tablet, wet granulation.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY
EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY MIXTURE
OF PIOGLITAZONE HCl AND METFORMIN HCl
Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa2
1Fakultas Farmasi Universitas Muslim Indonesia 2Sekolah Farmasi Institut Teknologi Bandung Abstract
This study was aimed to identify the physical interaction between PGZ-MFN by cogrinding. Solid state interaction was observed by cold contact method and phase diagram formation. The solid state grinding (SSG) and solvent drop grinding (SDG) was conducted on binary mixtures. The identification resulted in a binary system was characterized by differential thermal analysis (DTA), polarization microscopy, scanning electron microscope (SEM) and powder X-ray diffraction (PXRD). Furthermore, the solubility and dissolution testing of PGZ performed on the physical interaction results. The observation under the polarizing microscope showed that the new crystal habit was not found. The mixture has melting point lower than MFN and PGZ. This phenomenon was then confirmed with phase diagram arranged by thermogram of PGZ-MFN. That was identified mixture binary equimolar as eutectic mixture with eutectic temperature 187 °C. Meanwhile, PXRD data at equimolar mixture did not showed the new interference peak. The DTA and powder X-ray diffraction data of the equimolar solid compounds obtained from several tehnique showed similar result. The thermogram of all treated sampels had similar endothermic curve (185- 186C), and identical interference peaks of PGZ-MFN of 2θ 8.6; 12.2; 12.8; 17.2; 18.6; 22.7; 23.2 . Conclusion Based on the results of morphological analysis, the PXRD data and thermal properties, PGZ-MFN equimolar mixture showed the formation of a eutectic mixture.
Keywords: Cogrinding, physical characteristic, binary system, pioglitazone HCl, metformin HCl.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA
INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD
1Department of Pharmacy, Faculty of Mathematics and Natural Science, University of Sriwijaya Inderalaya, Indonesia *E-mail: [email protected] Abstract
The research had been performed to incorporate rifampin into PLGA submicron-sized particles. This research has a prospect to be applied to overcome the ineffectiveness use of rifampin for tuberculosis patients as rifampin was not stable in human lung macrophages, while Mycobacterium tuberculosis was able to survive in human lung macrophages. Rifampin was incorporated into submicron particles of PLGAs using the emulsion solvent diffusion method. The use of rifampin 50 mg in every batch resulted in the submicron-sized particles of 220 nm, PDI 0:12, zeta potential 21 mV and EE 37%. In the batch using rifampin 300 mg, resulted the submicron-sized particles of 410 nm, PDI 0:22, zeta potential 14 mV and EE 40%. The surface of the particles was visualized by SEM and hydrodynamic size compared to TEM. It was known that particle is spherical with a smaller diameter than the hydrodynamic size. TEM measurement revealed the size of particles with PVA was 208 nm. Keywords: characterization, PLGA, rifampin, hydrodynamic-size, TEM, %EE.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST
AGAINST Staphylococcus aureus OF KECOMBRANG
(Etlingera elatior (Jack) R.M.Sm.) FLOS EXTRACTS
Lilis Handrayani1, Ratih Aryani1*, Indra1
1Pharmacy Study Programme, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya Abstract
The formulation of kecombrang flos extract (Etlingera elatior (Jack) R.M.Sm.) liquid bath soap has been established. The objective of this research was to formulate liquid bath soap of kecombrang flos extract (Etlingera elatior (Jack) R.M.Sm.) and to test its antibacterial activity to Staphylococcus aureus. Kecombrang flos extract was extracted by maceration method using 96% ethanol, and followed by minimum inhibitory concentration (MIC) test using hole method. Concentration variation of kecombrang flos extract was conducted as F1 (6%), F2 (8%), and F3 (10%). The formula of liquid bath soap of kecombrang flos extract was evaluated using several examinations such as organoleptic, pH, viscosity, density, foaming stability, antibacterial activity test, irritation test and hedonic test. The result shows the liquid bath soap of kecombrang flos extract F1, F2 and F3 can inhibit the growth of Staphylococcus aureus. Based on statistical test using SPSS 21 (for trial) ANOVA method continued by LSD shows that F0 (negative control), F1, F2, F3 and positive control (triclosan 2.5%) have difference meaningful result with significance value < 0.05. Keywords: Kecombrang flos extract, Etlingera elatior, liquid bath soap, antibacterial,
Staphylococcus aureus. ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS
ANTIOXIDANT IN LOTION DOSAGE FORM
Sholichah Rohmani1*
1Universitas Sebelas Maret Solo, Indonesia *E-mail: [email protected] Abstract
The purpose of this research is to formulate lotion containing extract of eggplant peel as an effective and safe antioxidant lotion. Previous research showed that the anthocyanin in peel eggplant has antioxidant activity against free radical that cause aging on the skin. Extract of eggplant peel was obtained by maceration process using ethanol 70%. The extract was added in various concentrations in lotion formulation i.e. 0%, 0.5%, 1%, 2% and 3% based on the antioxidant activity that was determined using DPPH method. The produced lotions were characterized for the antioxidant activity, stability and physical properties including organoleptic, viscosity, pH and panellist acceptability. The result showed that antioxidant activity rated weekly and decreasing viscosity from week 0 to 8, pH of 5.62-6.93 was observed. Formulae I was the most acceptable lotion by pannelist.
Keywords: peel eggplant, lotion, antioxidant.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL TECHNOLOGY
OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND
SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION
Raymond Harris Mustafa1*, Lannie Hadisoewignyo1, Martha Ervina1, Lisa
Soegianto1, Wahyu Dewi Tamayanti1
1Faculty of Pharmacy, Widya Mandala Surabaya Catholic University Surabaya, Indonesia *E-mail: [email protected] Abstract
Sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygium polyanthum), which are effective to reduce blood sugar level with different mechanism, have been suggested to produce a synergy as antioxidant and anti-inflammatory agent, hence the optimum combination formula is remained to be elaborated. By reducing blood sugar level and showing antioxidant, and anti-inflammatory activity, both plants were hypothesized of its ability for ameliorating diabetes mellitus complexicity. This study aimed to discover the optimal combination formula of sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygium polyanthum) water fraction to produce anti-inflammation effect. Carrageenan was used to induced the inflammatory condition. Optimization was conducted by factorial design utilising 2 factors and 2 levels. Both factors, sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygium polyanthum) in the range of low level 1:10 and high level 10:1 with the used doses were 100 mg/kg BW at low level and 300 mg/kg BW for high level. The observed parameters were anti inflammation potential percent and edema rate. Conclusively, this study proposed that optimum combination formula of sambiloto herbal : salam leaves water fraction = 1.14 : 9.87 with 234 mg dose. The combination formula was theoretically resulted the optimum anti inflammation potential percent of 45.68%, and ER of 38.39% compared with other combination formulas. Keywords: salam leaf, sambiloto herbal, water fraction, anti inflammation, factorial design.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT
Arifah Sri Wahyuni1*, Rima Munawwaroh1, Esthi Utaminingsih1, Novita Sari1
1Faculty of Pharmacy University Muhammadiyah Surakarta Surakarta, Indonesia *Email: [email protected] Abstract
Natural hypoglycaemic compounds may be attractive alternatives to synthetic drugs or reinforcements to currently used treatments. Black rice bran is one of natural compound that containing anthocianins. The major anthocyanins component isolated from black rice bran is cyanidin 3-glucoside. In this experiment, fiveteen male rat were randomly allocated into 5 equal groups. They were induced by alloxan (150mg/kgbw, i.p) except Group I). If the blood glucose levels reach 200 mg/dL, they were given aquadest (Group II), black rice bran extract (BBE) respectively 50, 100 and 200 mg/kg.bw (Group III-V). Blood glucose level was analyzed at 4, 7, 10 days treatment. Insulin level was determined enzyme linked immunosorbent assay (ELISA). The result showed that baseline levels of blood glucose were statistically not different among the fivegroups (p>0.05). By the end of experiment BBE dose 50 mg/kgbw did not reduce blood glucose levels after 10 days of treatment (p>0.05). BBE dose of 100 and 200 mg/kgbw decreased blood glucose levels. The level of blood glucose after administered 10 days by BBE 200 mg/kgbw was 131.33 ± 8.08 mg/dL. The results showed that insulin levels in diabetic rats increased by administration of the extract at dose 200 mg/kg b.w was 15,20±9,5ng/mL. It can be concluded that BBE can be therapeutically helpful as antidiabetic agents
Keywords: cyanidin 3-glucoside, black rice bran extract, antidiabetic.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF
SAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN
(Phyllanthus niruriL.)IN ALLOXAN- INDUCED DIABETIC RATS
Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da'i1,
Doni Wibowo1
1 Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail : [email protected] Abstract
Combination of an oral antidiabetic with antioxidant is potential to treat disease and the complications of diabetes mellitus. The aim of this study was to investigate the antioxidant activities of nanoemulsion containing extract of sambiloto (Andrographis paniculata (Burm F.) Ness.) and meniran (Phyllanthus niruri, L.) (NESM) in alloxan-induced diabetic rats. Rats were divided into 6 groups that were orally administered by nanoemulsion base (NE), vitamin E (100 mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract of sambiloto and meniran (ESM) (400 mg/kg) for 14 days. Blood was withdrawn on day 0, 1, and 14, and malondialdehyde (MDA) level was determined using UV-Vis spectrophotometer. The result showed that increasing dose of NESM reduced plasma MDA levels compared to NE, vitamin E and ESM groups. It can be concluded that NESM possess antioxidant activities.
Keywords: nanoemulsion, Andrographis paniculata, Phyllanthus niruri, malondialdehyde
(MDA), antioxidant. ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO (Andrographis
paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS EXTRACT
IN WISTAR RATS
Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da'i1,
Febby Lovita Sari1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *Email: [email protected] Abstract
Andrographis paniculata leaves (AL) and Phyllanthus niruri herbs (PH) have been proven scientifically to have antidiabetic and antioxidant activities. Nanoemulsion system has been developed containing of both AL and PH extracts, using Tween 80 and polyethylene glycol as surfactant and cosurfactant, respectively. The present study is to investigate the acute toxicity of the nanoemulsion on Wistar rats. Five groups of rats were orally treated with nanoemulsion doses of 31.25, 125, 500, 2000 mg/kg and control. General behavior, adverse effects and mortality were observed for up to 14 days. Oral administration of nanoemulsion at the highest dose of 2000 mg/kg resulted in no mortalities or evidence of adverse effects, indicated that nanoemulsion is nontoxic with a LD50 higher than 2000mg/kg. Throughout 14 days of the treatment, there was no significant change in behavior and body weight of rats in both treatment and control groups. Histopalogical study showed that there were congestion in the liver and inflammation in the kidney by the highest dose of 2000 mg/kg. In addition, the pancreas and gastric of rats in the highest dose group showed a significant change compared to the control group.
Keywords: acute toxicity, nanoemulsion, A. paniculata, P. niruri, herbal extract.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY
ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii
(Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)
Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina1
1Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya University South Sumatera, Indonesia Abstract
Christmas palm (Adonidia merrillii (Becc.) Becc.) contains flavonoids, tannins, triterpenoids, and steroids. Antioxidant activity of christmas palm has been investigated. Based on research, antioxidant has a potential as an anti-inflammatory agent. Christmas palm seed extract anti-inflammatory activity test with rat paw edema method which induced by carragenaan has been done. Ethanolic, ethyl acetate, and n-hexane extract of christmas palm were divided into three dose groups, 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW. Negative control was CMC Na in distilled water and positive control was 0.82 mg/200 g BW diclofenac Na. Thirty three Wistar male rats as test subjects were divided into 11 groups (1 negative control group, 1 positive control group, and 9 treatment groups). Anti-inflammatory activity was tested by measuring the volume of rat paw edema using plethysmometer. The test results showed the average of inhibition percentage of the ethanolic, ethyl acetate, and n-hexane extracts were 46.05%; 29.95%; and 34.63% respectively. This shows that the ethanolic extract has better inflammation inhibitory activity than ethyl acetate and n-hexane extracts. Non-parametric statistical Mann Whitney test of 800 mg/kg BW ethanolic extract at 30' and 60' showed that both were not significantly different (p>0.05) to diclofenac Na. Christmas palm ethyl acetate extract has anti-inflammatory activity far below diclofenac Na, but its effect was constant until 150'. n-hexane extract of 800 mg/kg BW at 60' showed anti-inflammatory effect that was almost equivalent to diclofenac Na (p>0.05). Christmas palm seed extract ED50 has been calculated based on the average of edema inhibition percentage in rats. ED50 of christmas palm ethanolic extract is 640.42 mg/kg BW.
Keywords: Adonidia merrillii (Becc.) Becc., anti-inflammatory, rat paw edema, diclofenac Na.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus
oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF MALE WISTAR RATS
BLOOD INDUCED BY PARACETAMOL
Intan Martha Cahyani1*, Bekti Nugraheni1
1Sekolah Tinggi Ilmu Farmasi "YAYASAN PHARMASI" Semarang, Indonesia Abstract
Indonesia is a country with a high prevalence of liver disease. Liver is important for survival and plays a role in every metabolic function of the body. One of the causes of liver disorder is because of drugs. One of the drugs that cause liver damage is paracetamol. Glucomannan is a major component of the porang tuber that serves as a soluble fiber. Glucomannan is thought to have hepatotherapy effects as a potential antioxidant. The aim of this study is to determine the effect of glucomannan porang tuber (Amorphophallus oncophyllus Prain ex Hook. F.) with graded doses on the blood levels of SGPT and SGOT wistar male rats induced by paracetamol dose of 1,638 g/kg BB. This research was experimental study. The treatment in this study was porang glucomannan with a dose of 25 mg / kg, 50 mg / kg and 100 mg / kg. The Population of the study was white male Wistar rats aged 3-4 months with a weight of 180g - 250g, and healthy. The result of the analysis showed that glucomannan Porang (Amorphophallus oncophyllus Prain ex Hook. F.) has an effect as hepatotherapy. Giving glucomannan porang at a dose of 50 mg / kg rat has an effect to decrease blood levels of SGPT and SGOT wistar male rats induced by paracetamol.
Keywords: Porang tuber (Amorphophallus oncophyllus Prain ex Hook. F.), SGPT, SGOT.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY
THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OF
THYMI HERBS (Thymus vulgaris [L.])
Ken Inayati Latifa1*, Tanti Azizah Sujono1, Ika T. Dian Kusumowati1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail: [email protected] Abstract
Oxidative stress occurs because of an imbalance between oxidants and antioxidants which are then potentially cause damage to cells and reportedly play an important role in the process of liver damage. Free radicals can increase lipid peroxidation, which will then be decomposed into malondialdehyde (MDA) in the blood. MDA is a marker of cell damage caused by free radicals. Extract of Thymi contains chemical compounds such as terpenoids, flavonoids, aglycone, and phenolic acids. The content of flavonoid compounds contained in herbal Thymi can serve as an antidote to the radical. The purpose of this study is to determine the effect of herbal extracts Thymi in rats MDA levels. The study used 20 rats were divided into 4 groups. Group I was a normal control group was given distilled water of 2.5 ml/kg, while group II-IV were given Thymi herbal extract for 5 days with a dose of 50, 100, and 150 mg/KgBW, respectively. Blood was drawn for MDA content measurement using spectrophotometer at a wavelength of 530 nm. The results showed that herbal extracts Thymi can reduce levels of MDA in groups II, III, and IV. Meanwhile, there was also a decrease of MDA level in grup 1. So in this study decreased levels of MDA which occurs in group II, III, and IV can not be said as a result of administration of herbal extracts Thymi due to a decrease in MDA levels also occurred in group I were only given distilled water.
Keywords: Thymus vulgaris, MDA (malondialdehyde).
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY
PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE 2
DIABETIC RATS
Kun Sri Budiasih1*, Kartika Ratna Pertiwi1
1Faculty of Mathematics and Natural Sciences, Yogyakarta State University Yogyakarta, Indonesia *E-mail: [email protected] [email protected] Abstract
The chromium (III)- amino acid complex based hypoglicemic agent was investigated on nicotinamide-streptozotocin induced diabetic Wistar rats. The rats were divided into 7 groups each consist of 4 animals. Three groups are control (+) with chromium picolinate (CrPic), control (-) diabetic group (DM), and control non diabetic (non DM). Furthermore, three groups were examined on the effect of Cr-AA[Cr(µ-OH)(glu)(OH)2]2·6H2O at dose of (50, 150 and 300 µg/day). In addition, the last group was studied on the effect of control group by glibenclamide. The blood glucose levels were measured before and after treatment. The results show that supplementation of Cr(III)-complex in 8 weeks decreased the blood glucose level in the range of 46.446 – 79.593 %.
Keywords: hypoglicemic, chromium (III), amino acid, complexes, nicotinamide-streptozotocin,
diabetic rats.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITE OF
MANGOSTEEN (Garciniamangostana L.) LEAF
Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita1
1Faculty of Pharmacy, Widya Mandala Catholic University Surabaya Surabaya, Indonesia *E-mail: [email protected] Abstract
Mangosteen (Garcinia mangostana L.) is a tropical plant which its fruit peel is widely used as an antioxidant, anti-diarrhea, anti-inflammatory, antitumor, and as an antibacterial. The previous study found the antibacterial activities of extract and metabolites of endophytic fungi of mangosteen rind. This research was aimed to explore utilization of mangosteen leaf and to screen antibacterial potency of the mangosteen leaf endophytic fungi metabolites. Endophytic fungi were isolated from leaf of mangosteen in the Malt Extract Agar (MEA) medium in order to get 2 colonies of endophytic fungi. Screening of potential antibacterial metabolites was assessed using diffusion method and bioautography, obtained results of the antibacterial activity against Escherichia coli (Ec) and Staphylococcus aureus (Sa) of the metabolites endophytic fungi of leaf. Macroscopic and microscopic characteristics from fungi isolate which has antibacterial potency, was observed and fermented into Potatoes Dextrose Yeast (PDY) medium for 14 days. At day 14, biomass and supernatant were separated and carried out separation by liquid-liquid extraction. The supernatant and biomass were fractionated using n-hexane, ethyl acetate, and water. Each fraction was eluated to several mobile phase and tested its antibacterial activity against Ec and Sa. The result showed that there is a potential antibacterial activity of endophytic fungi metabolites leaf ED2 against Sa. Bioautography result was observed that the compound has antibacterial activity and is supposed as flavonoid compounds. It was supposed that endophytic fungi ED2 was a group of Trichoderma.
Keywords: mangosteen (Garcinia mangostana L.), endophytic fungi, antibacterial activity,
Escherichia coli, Staphylococcus aureus. ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis)
AND BLACK CUMIN (NIGELLA SATIVA) AGAINST XANTHINE OXIDASE INHIBITION
ON HYPERURICEMIC MICE
Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail: [email protected] Abstract
The combination extracts of tempuyung (Sonchus arvensis) and black cumin (Nigella sativa) can decrease uric acid levels on the previous result research. However, the mechanism of decreasing uric acid levels was unknown certainly. This study aimed to determine the inhibitory effect of xanthine oxidase by the combination extracts of black cumin and tempuyung on hyperuricemic mice. Hyperuricemic mice were induced by 250 mg/kgBW potassium oxonate was given one hour before treatment. The mice were divided into 3 groups, group I was given 10 mg/kgBW allopurinol (positive control), group II was given 0.5 mL/20gBW aquadest (negative control) and group III was given the combination extracts of black cumin-tempuyung with dose 200 mg/kgBW during 4 days administration. The supernatant of liver was taken and measured of xanthine oxidase levels by spectrophotometer UV at 290 nm. The data of xanthine oxidase activity were analyzed by Kruskal-Wallis and Mann-Whitney method. Xanthine oxidase activity of the combination extracts of blackcumin-tempuyung was 4.54±0.9 U/mg, very significantly than control negative was 8.00±0.22 U/mg (p<0.05). Inhibition of xanthine oxidase by the combination extracts of black cumin-tempuyung was 43.26±11.29%, lower than allopurinol was 90.19±0.36%. Keywords: Hyperuricemic, xanthine oxidase inhibition, Sonchus arvensis, Nigella sativa.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND
ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST
Escherichia coli AND Shigella sonnei
Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail: [email protected] Abstract
Chloramphenicol, a broad spectrum antibiotic, is used to treat several bacterial infections. However, it has several side effects such as headache, rash, diarrhea, nausea, vomiting, bone marrow suppression, and aplastic anemia. Combining antibiotic with another antibacterial agent may decrease the dose thus the side effects. This study aimed to investigate the antibacterial activity of chloramphenicol combined with ethanolic extract of pacar air (Impatiens balsamina) leaves against Escherichia coli and Shigella sonnei. Chloramphenicol (30 µg), extract (2500 µg), combination of chloramphenicol and extract (22.5 µg + 625 µg and 15 µg + 1250 µg), dimethylsulfoxide, and water for injection were tested for antibacterial activity against Escherichia coli and Shigella sonnei using well diffusion method. Data was analyzed using Mann-Whitney test.The results showed that only chloramphenicol (22.5 µg) combined with extract (625 µg) has inhibition zone diameter, which was not significantly different from chloramphenicol alone (30 µg) when it tested against E. coli. This result indicated that combination of the antibiotic in lower concentration and extract can achieve the same antibacterial activity as antibiotic alone in higher concentration.
Keywords: antibacterial, chloramphenicol, Impatiens balsamina, combination.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVES AND
ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY PROPYLTIOURACIL
AND HIGH FAT DIET
Tanti Azizah Sujono1*, Haryoto1, Ratna Kartikasari1, Laily Ieda Quntari1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia Abstract
Empirically Murbei leaves are used to treat diseases such as hypercholesterolemia. Murbei leaves contain quercetin 3- (6-malonylglucoside) and rutin which shows a strong inhibitory effect on LDL oxidation. This study aimed to verify the effect of the ethanol extract of murbei leaves in lowering total cholesterol in hyperlipidemic rats and the effect of combination extract murbei with simvastatin to the hypocholesterolemic effect of simvastatin. Twenty four Wistar male rats were divided into 6 groups randomly, group I-VI were given high fat diet and (propyltiouracil) PTU to induce hypercholesterolemia. Group I as positive control was treated simvastatin 3.6 mg/kgbw, group II as a negative control was given CMC Na, group III-V were given ethanol extract of murbei leaves orally at a dose of 0.4; 0.6; and 0.8 g/kgbw respectively, group VI were treated a combination of murbei extract 0.4 g/kg with simvastatin. Total cholesterol was measured using a spectrophotometer visible (λ = 500 nm) with CHOD-PAP method as many as 3 times, i.e on day 0 (baseline), 14 days after induced hypercholesterolemia with PTU and high-fat-diet and 14 days after treatment of murbei extract with still be given PTU and a high-fat diet. The results showed that murbei leaves extract dose 0.4; 0.6 and 0.8 g/kg were able to reduce total cholesterol with percent decrease in cholesterol levels by 32.94 ± 10.07; 40.17 ± 4.61 and 45.71 ± 4.27% respectively. Ethanol extract of murbei 0.4 g/kg can increase hypocholesterolemic effect of simvastatin when used in combination (p< 0.05). Keywords : Murbei (Morus alba L), antihypercholesterolemic, propyltiouracil, high fat diet.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus
polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITH
WRITHING REFLEX METHOD
Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia Abstract
Red Dragon fruit (Hylocereus polyrhizus) is one of the plants that grow in Indonesia. Peel of fruit (Hylocereus polyrhizus) contain oligosacharida and Betasianin. Oligosacharida showed activity as prebiotic, which can lower the resistance to acidic conditions in the stomach, meanwhile betasianin can protect the cells from damage caused by free radical. This research aims to prove whether peel of Dragon fruit has activity as analgesic in mice. Twenty five mice were divided into 5 groups. group I was given Na CMC 1% (negative control), group II was treated paracetamol 65 mg/kgBW (positive control), group III, IV, and V were given ethanolic extracts of dragon fruit peel at dose 0.25, 0.5, and 1 g/kgBW respectively. Twenty minutes later, all mice were induced pain by 0,1 ml intraperitoneal acetic acid 1% and the cumulative of writhing reflect of mice were calculated for one hour. Then percent inhibition of writhing were analyzed by Kruskal Wallis test and continued by Mann-Whitney with 95% confidence level. The results showed that ethanolic extracts of dragon fruit peel showed analgesic effect. It can decrease the number abdominal constrictions and also increased the percentage inhibition of writhing at dose of 0.25, 0.5, and 1 g/kgBW with percent of inhibition 42,76 ± 2,04; 1.42 ± 49,32; and 61,38 ± 1,37% respectively.
Keywords: Hylocereus polyrhizus, Dragon fruit peel, analgesic, writhing reflect
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED
OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS
Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja2
1Faculty of Pharmacy, Universitas Ahmad Dahlan 2Faculty of Medicine, Universitas Gadjah Mada Yogyakarta, Indonesia *E-mail: [email protected] Abstract
Oxidative stress, an imbalance between free radicals and the antioxidant system, is known to contribute to the pathogenesis of the development of dementia. Ethanolic extract from turmeric (Curcuma longa L.) containing the curcumin constituent has been reported to produce antioxidant effects. This study aims to determine the effect of turmeric extract on markers of oxidative stress in Spraguey Dawley rat induced by (trimethyltin) TMT. Thirty six adult male Sprague-Dawley rats (195-215 g) were divided randomly into six groups consisting of 6 rats for each group. The rats were divided randomly into six groups, i.e.: N group, which served as a normal control group; T group, which was given intra-peritoneal injection of TMT chloride; T-Cit group, which was treated with oral citicoline and TMT chloride injection; and three T-TE groups, which were treated with three different dosages of oral turmeric rhizome extract, as well as TMT chloride.The turmeric rhizome extract and citicoline solutions were given at day 1 up to day 28 of experiment, whereas the TMT chloride injection given as a single dose of 8 mg /kg bw was administered at day 8 of experiment. At day 36 the blood were taken for plasma MDA level determination. Afterthat all rats were sacrificed and the cerebral hemisphere were then dissected out from the skull. The left cerebral hemispheres were used for biochemical observation i.e. MDA and GSH level, activities of SOD, catalase (CAT) and GPx. The turmeric extract dose of 200 mg/kg bw could prevent oxidative stress induced by TMT through the decrease of levels of plasma and brain MDA and increased the activities of SOD, CAT, GPx, and the level of GSH of brain. These effects seem to be comparable to those of citicoline.
Keywords: Curcuma longa L., trimethyltin, oxidative stress.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACOLOGY AND MICROBIOLOGY ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF
SAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN
(Phyllanthus niruri L.) IN ALLOXAN-INDUCED DIABETIC RATS
Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da'i1,
Fajar Kholikul Amri1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail : [email protected] Abstract
Preparation of nanoemulsion containing combination of extract of A. Paniculataand P. Niruri (NESM) has been successfully developed. The present study was carried out to investigate the antidiabetic activity of the nanoemulsion preparation in alloxan-induced diabetic rats.Rats were divided into 6 groups that were given oral administration of nanoemulsion base (NE), glibenclamide (5 mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract of sambiloto and meniran (ESM) (400 mg/kg) for 14 days. The hypoglycemic effect was measured by blood glucose level. Oral administration of the NESM at a dose of 100, 200, and 400 mg/kg daily for 14 days showed a significant (P<0.05) decrease in blood glucose level as compared with the diabetic rats. The results indicated that the nanoemulsion preparation possesses significant antidiabetic activity.
Keywords: nanoemulsion, Andrographis paniculata (Burm F.) Ness., Phyllanthus niruri, L.,
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" CLINICAL AND COMMUNITY PHARMACY
OVERVIEW ABOUT ANTIBIOTIC'S PRESCRIBING IN URINARY TRACT INFECTION
ROEMANI SEMARANG HOSPITAL'S INPATIENTS
Hening Pratiwi1*
1Studied Program of Pharmacy UNSOED Purwokerto, Indonesia *E-mail: [email protected] Abstract
Inappropriate antibiotics prescribing in Urinary Tract Infection (UTI) can lead antibiotic's resistance. Therefore, hospitals should have a formulary as a reference for providing medical services to the patients. This study aims to determine the types of antibiotics that prescribed for UTI treatment on January until November 2009 and determine the level of antibiotics prescribing conformity with the Roemani Semarang hospital's formulary and WHO 2001 guidelines. This study used a non-analytical descriptive design and retrospectively. The samples were 73 patients. This study includes the pattern of antibiotic prescribing in UTI patients and conformity with 2009 hospital formulary and 2001 WHO guidelines. The results showed that antibiotics are widely used cefotaxime (cephalosporins) 14 cases (24%), levofloxacin (quinolones) 11 cases (18%), and ceftriaxone (cephalosporins) 10 cases (17%). The combination that widely prescribed are cephalosporins combination with quinolones 3 cases (21%), cephalosporin combination with other cephalosporins 3 cases (21%), combination of cephalosporin with an aminoglycoside 2 cases (14%), there are 68 prescriptions (93%) suitable with hospital formulary, and 5 prescriptions (7%) not listed on the formulary of Roemani hospital 2009. The UTI antibiotic monotherapy in women, men, and children do not exist in accordance with the WHO guidelines 2001.
Keywords: Urinary Tract Infections, antibiotics, hospital formulary, RS. Roemani Semarang.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" CLINICAL AND COMMUNITY PHARMACY RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA IN
ADULT PATIENTS IN THE INPATIENT INSTALLATION OF HOSPITAL "X"
SURAKARTA IN 2014
Ida Ayu Pebrina1*, Suharsono1, Suprapto1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail: [email protected] Diarrhea is still a public health problem in developing countries such as Indonesia, due to its morbidity and mortality are still high. Antibiotics are used to attack microbial infection and kill or inhibit the growth of bacteria. The purpose of this study is to determine the rationality of the use of antibiotics in adult patients with diarrhea in the inpatient installation of hospital "X" Surakarta in 2014. The study used non-experimental method. The study used 46 medical records of patients using purposive sampling technique. The instrument that used is the data collection sheets and the medical records of diarrhea patient as the material. From the data that has been captured and processed, the rationality of antibiotic therapy for the treatment of diarrhea include: precise indication of the percentage of 100 % , right drug as much as 97.82%, right dosage as much as 97,82% , proper frequency and duration of drug administration as much as 78.26%.
Keywords: antibiotics, diarrhea, adult patient.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" CLINICAL AND COMMUNITY PHARMACY CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY
ON CHILDREN WITH TYPHOID FEVER
Rasmaya Niruri1*, N.P. Yulia Purnami1, Julius D.Tansale2,3, I.B.Eka Erlangga3
1Department of Pharmacy, Faculty Math and Science, Udayana University 2Petri Bali, Bali 3Puri Raharja Hospital, Denpasar, Bali, Indonesia Abstract
The aim of this research is to evaluate clinical recovery time on children with typhoid fever (TF) after receiving cephalosporin. This cross-sectional research was conducted in Puri Raharja Hospital. Data of 12 TF clinical features was collected from all pediatric patients with TF on the period of January 1st, 2013 to March 31st, 2014, who received the same antibacterial medicine during hospitalization. A patient, who had fever clearance and showed general well-being, was considered having a good clinical-response from TF. A patient was discharged after showing a good clinical therapeutic response. Seventy nine from 89 subjects were prescribed with cefotaxime, and the rest got ceftriaxone (9 patients) and cefixime (1 child). Length of stay in the hospital (median [time range] in days) were 5 [4-10] with cefotaxime; 4 [4-7] with ceftriaxone; and 7 [7] with cefixime. All of 89 children showed good clinical-response to the medicines. The majority of children were free from TF symptoms when they were discharged, but not from weakness- fatigue.
Keywords: clinical improvement, children, typhoid.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" CLINICAL AND COMMUNITY PHARMACY TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND
6-MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA
Rasmaya Niruri1*, K. Trisna Komalasari1, Ketut Ariawati2
1Department of Pharmacy, Faculty of Math and Science, Udayana University, Bali 2, Division of Hematology-Oncology, Department of Pediatric, Sanglah Hospital, Bali Bali, Indonesia Abstract
The objective of this research was to evaluate the level of AST and AST before and after consolidation phase of Indonesian Protocol (2006) Chemotherapy on acute lymphoblastic leukemia (ALL).This cross sectional research was conducted in Sanglah Hopital. All hospitalized children (0-12 tahun) in the period of January 2012 – May 2014, who got high dose methotreaxate (HD MTX) and 6-mercaptopurin (6-MP) and had normal AST-ALT level before receiving HD -MTX- and 6-MP, were included. Thirteen of 39 patients who met the criteria was obtained, but only ten patients, who had complete AST-ALT data on week 8th, 10th, and 12th of consolidation phase. From 10 children who had the complete data, four of them (who didn't receive Ursodeoxycholic acid (UDCA)) had the highest transaminase enzyme level at week 12th and the rest 6 patients showed declining AST-ALT level after receiving UDCA. Keywords: transaminitis, HD-MTX, 6-MP, children, ALL.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" CLINICAL AND COMMUNITY PHARMACY TREATMENT AND COST ANALYSIS OF DIARRHEA PATIENTS OF INPATIENT
INSTALLATION OF RSUD dr. MOEWARDI SURAKARTA BY BPJS PROGRAM IN 2014
Saktya Ayu Donna P1*, Suharsono1, Suprapto1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail: [email protected] Abstract
Diarrhea or gastroenteritis is still a major disease in developing countries that can lead to death. This research was aimed to analyse the cost and treatment of gastroenteritis's patients of inpatient installation using BPJS program at RSUD Dr. Moewardi in 2014. This research used descriptive, non-experimental, and retrospective method by collecting data from the medical report of patients. There are 28 cases fulfil inclusive criterion. Cost was analysed including average total cost, prescription, medical stay, laboratory, visit, action and health tools and administration cost. The result showed that (a) Average total cost of diarrhea therapy every class treatment: VIP class is Rp.3.239.007 ± Rp.1.914.830, class I is Rp.1.522.475 ± Rp.0, class II is Rp.2.964.331 ± Rp.368.155; class III Rp.2.043.954 ± Rp.1.716.563. (b) class treatment with most cheap is class III is Rp.2.043.954 ± Rp.1.716.563. Stay care diarrhea's patient of BPJS at RSUD Dr. Moewardi year 2014 uses medicine groups are rehydration liquid (100%) RL is 17 cases (65.38%) and asering is 9 cases (34.62%). Antibiotic groups are 81 patients (81%), adsorben group is 75 cases (75.0%), antiulcer kidney group is 16 cases (19.75%), antiemetic is 9 cases (11.11%), analgetic-antipeuritic 9 cases (11.11%) supplement food is 9 cases (11.11%) and probiotic is 1 case (1.23%).
Keywords: cost analysis, RSUD dr. Moewardi, diarrhea.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL CHEMISTRY ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF PARKIA SEED AND POD
(Parkia speciosa Hassk.) BY DPPH (1,1-DIPHENYL-2- PICRYLHYDRAZYL) METHOD
Indri Kusuma Dewi1*, Agus Winarso1
1Department of Jamu Poltekkes Kemenkes Surakarta Klaten, Indonesia *E-mail: [email protected] Abstract
Parkia speciosa is one of plants that usually used for food materials. Seed and pod of Parkia speciosa contain flavonoid that act as antioxidant. The aim of this study was to examine antioxidant activity at ethyl acetate extract of seed and pod Parkia speciosa tha were expressed by Inhibitor Concentration 50 (IC50). Testing of antioxidant activity was done by DPPH method (1,1-dipheny-2-picrylhydrazyl) using spectrophotometer UV-Vis at wavelength 518 nm. The result showed that ethyl acetate extract of the seed of Parkia speciosa had low antioxidant activity with the IC50 value of 274.702 ppm, while ethyl acetate extract of the pod Parkia speciosa did not show any antioxidant effect with the IC50 value of 685.857 ppm.
Keywords: Parkia speciosa, seed, pod, antioxidant, DPPH.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL CHEMISTRY A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL
AZIDES AS A VERSATILE APPROACH TO GENERATE NEW PHARMACOLOGICALLY
ACTIVE COMPOUNDS
Muhammad Aswad1, Junya Chiba1*, Yasumaru Hatanaka1, Takenori Tomohiro1
1Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama Sugitani, Toyama, Japan *E-mail: [email protected] Abstract
Several click-type reactions have been developed and applied to biological conditions, however, the ligations generally need some catalysts or additives for their practical use. We recently reported a chemoselective reaction between thioamides and sulfonyl azides to afford sulfonyl amidines in the absence of any activation additives.1 The reaction proceeded by mixing the thioamide and sulfonyl azide at room temperature in various solvents, and water exhibited the highest performance with respect to efficiency. The characteristics of amidines within the product framework are polar and hydrophilic, so that we applied this reaction for the simple derivatization of sugars, such as nojirimycin, to develop novel inhibitors for glucosidases. A cyclic thioamide derivative of nojirimycin could be synthesized from gluconolactone derivatives.2By coupling of the thioamide with several sulfonyl azides, the chemoselective ligation successfully afforded the corresponding amidine compounds, which are the potential candidates as novel glycosidase inhibitors. ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" PHARMACEUTICAL CHEMISTRY ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROM
RIZHOSPHERE OF Zingiber officinale
Nanik Sulistyani1,2*, Yosi Bayu Murti3, Jaka Widada4, Mustofa5
1Faculty of Pharmacy, Universitas Ahmad Dahlan 2Doctoral Program, Faculty of Medicine, Universitas Gadjah Mada 3Faculty of Pharmacy, Universitas Gadjah Mada 4Departement of Microbiology, Faculty of Agriculture, Universitas Gadjah Mada 5Departement of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta, Indonesia *E-mail: [email protected] Abstract
Isolation of bacteria from Zingiber officinale rizosphere in Magelang, Central Java, Indonesia has been carried out and got many isolates in the previous study. One of them is isolate J4 having potencial activity as antibiotic producer. This study aims to identify the antibiotic produced by isolate J4. Research was performed with examining the antibacterial activity of ethyl acetate extracts of culture broth and their fractions using cup-plate method as well as bioautography. The active fractions were then analysed using IR Spectroscopy. Identification of selected bacteria was based on the 16S rRNA gene sequence and the active fraction was characterized by LC-TOF-MS to identify the molecular mass of compounds contained in the fractions. Result showed the isolate J4 revealed antibacterial activity of both extracts and their fractions. The active fraction is the chloroform-methanol (7:3) fraction. According to its IR spectra, there was detected OH and CH stretching vibration as well as carbonyl stretching. Based on the LC-TOF-MS, the active fraction contains 3 compounds with molecular mass of 270, 274 and 404. The BLAST of 16S rRNA sequence revealed that isolate J4 is Burkholderia sp.
Keywords: antibiotic, isolate J4, soil bacteria, Burkholderia sp.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" MOLECULAR BIOLOGY CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION OF
ETHANOL EXTRACT OF SALA PLANTS LEAVES (Cynometra ramiflora Linn.)
AGAINTS WiDr CELL
Haryoto1*, Anis N. Irjayanti1, Tanti Azizah Sujono1, Muhtadi1, Andi Suhendi1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia *E-mail: [email protected] Abstract
Previous study reported that ethanolic extract of Sala plants leaves (Cynometra ramiflora Linn.) had cytotoxic activity against WiDr cells with IC50 of 6.37 μg/mL. This study aims to determine the cytotoxic activity of polar, semipolar, and nonpolar fraction of ethanolic extract of Sala plants leaves (Cynometra ramiflora Linn.) against WiDr cells, their IC50s, and compounds contained in each fraction of ethanol extract of Sala plants leaves. Fractionation was performed using Vacuum Liquid Chromatography method with silica G60 as stationary phase and n-hexane:ethylacetate (8:2, 7,5:2,5, 7:3,6:4, and 3:7) and ethanol as mobile phase. Compounds contained in each fraction was analysed using thin layer chromatography method with silica GF254 as stationary phase and mobile phase n-hexane:ethylacetate (7:3). Cytotoxic activity assay was performed using MTT assay method. Polar fraction of ethanol extract of Sala plants leaves has cytotoxic activity against WiDr cells with IC50 value of 231.953 μg/mL. Semipolar and nonpolar fractions do not show cytotoxic activity against WiDr cells. Doxorubicin was used as positive control and obtained IC50 of 1.721 μg/mL. The polar fraction contains of flavonoids, phenolics and alkaloids, while the non-polar and semipolar fractions contain phenolic compounds and alkaloids.
Keywords: Cytotoxic, fractionation, MTT assay, Cynometra ramiflora, WiDrcell, IC50.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" MOLECULAR BIOLOGY CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN
HeLa CELLS
Retno Arianingrum1*, Indyah Sulistyo Arty1
1Department of Chemistry Education, Universitas Negeri Yogyakarta Yogyakarta, Indonesia Abstract
Chalcones, a group of aromatic enones, is known have a variety of biological properties, including anticancer. We have synthesized a chalcone derivate 1-(4'-bromophenyl) - 3-(4-hydroxy-3-methoxyphenyl)-2-propene-1-on by aldol condensation reaction. The aim of the present study was to investigate the anticancer potency of the compound in HeLa cervical cancer cells by observing cytotoxic and apoptotic effect. The cytotoxic properties were determined using MTT assay and apoptosis induction was carried out using flowcytometry. The result indicated that the compound has cytotoxic effect on HeLa cells with IC50 of 53 M and was able to induce apoptosis. Hence, 1-(4'-bromophenyl) -3-(4-hydroxy-3-methoxyphenyl)-2- propen-1-on is potential to be developed as anticancer agent for cervix cancer by inducing apoptosis. However, the molecular mechanisms need to be explored further.
Keywords: chalcones derivate with Bromo substituens, cytotoxic, apoptosis, HeLa.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses" MOLECULAR BIOLOGY SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION
IN ESCHERICHIA COLI
Imam A. Wicaksono1, Tresna Lestari2*, Evi U. Ulfa3, Catur Riyani1, Elfahmi1
1Institut Teknologi Bandung 2STIKes Bakti Tunas Husada Tasikmalaya 3Universitas Jember Indonesia *E-mail: [email protected] Abstract
CYP71AV1 and ADS are two enzymes involved in artemisinin biosynthesis. In this research, sub-cloning of cyp71av1 and ads in pETDUET1 (pETDUET1_cyp/ads) has been done. The result of transformation has been confirmed by migration, restriction and sequencing analysis. Overproduction of CYP71AV1 and ADS was done at temperature 37 °C using 0.5 mM IPTG induction. The protein produced mostly formed as inclusion bodies, therefore the optimization of overproduction condition is still needed.
Keywords: CYP71AV1, ADS, pETDUET1, Sub-cloning.
ICB Pharma II – 31 October 2015 "Current Breakthrough in Pharmacy Materials and Analyses"
CV OF SPEAKER
Jackie Y. Ying received her B.E. and Ph.D. from The Cooper Union and
Princeton University, respectively. She joined the faculty at
Massachusetts Institute of Technology in 1992, where she was Professor of Chemical Engineering until 2005. She has been the
Founding Executive Director of the Institute of Bioengineering and
Nanotechnology in Singapore since 2003. For her research on nanostructured materials, Prof. Ying has been recognized with the
American Ceramic Society Ross C. Purdy Award, David and Lucile Packard Fellowship, Office of Naval Research Young Investigator Award,
National Science Foundation Young Investigator Award, Camille Dreyfus Teacher-Scholar Award, American Chemical Society Faculty Fellowship
Award in Solid-State Chemistry, Technology Review's Inaugural TR100 Young Innovator Award, American Institute of Chemical Engineers (AIChE) Allan P. Colburn Award, Singapore National
Institute of Chemistry-BASF Award in Materials Chemistry, Wall Street Journal Asia's Asian Innovation Silver Award, International Union of Biochemistry and Molecular Biology Jubilee
Medal, Materials Research Society Fellowship, Royal Society of Chemistry Fellowship, American Institute for Medical and Biological Engineering Fellowship, and Crown Prince Grand Prize in
the Brunei Creative, Innovative Product and Technological Advancement (CIPTA) Award. Prof. Ying was elected a World Economic Forum Young Global Leader, and a member of the
German National Academy of Sciences, Leopoldina. She was named one of the "One Hundred Engineers of the Modern Era" by AIChE in its Centennial Celebration. She was selected by The
Muslim 500 in 2012, 2013 and 2014 to be one of the world's 500 most influential muslims. She was selected as an Inaugural Inductee for the Singapore Women's Hall of Fame in 2014. She is
the Editor-in-Chief of Nano Today, which has an impact factor of 15.000.
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
CV OF SPEAKER
Full name: Takenori Tomohiro, Ph.D.
Current position: associate professor, Graduate School of Medicine
Education/Career: 1980: University of Tsukuba ( 1984)
1984: Graduate School of Pure and Applied Sciences, University of Tsukuba ( 1986)
1986: National Chemical Laboratory for Industry (reorganized to "National Institute of Advanced Industrial Science and Technology" :
1992: University of Oxford (UK) as a postdoctoral researcher ( 1994)
2002: Université Louis Pasteur (France) as a visiting professor
2002: Toyama Medical and Pharmaceutical University (reorganized to "University of Toyama")
Current publications:
1. Tomohiro, T., Morimoto, S., Shima, T., Chiba,
ligand-binding sites within protein. Chem.
J., Hatanaka, Y. An isotope-coded fluorogenic
Commun. 2013, 49, 1811-1813.
cross-linker for high-performance target
5. Tomohiro, T., Kato, K., Masuda, S., Kishi, H.,
identification based on photoaffinity labeling.
Hatanaka, Y. Photochemical Construction of
Angew. Chem. Int. Ed. 2014, 53, 13502-
Coumarin Fluorophore on Affinity-Anchored
13505.Tomohiro, T., Yamamoto, A., Tatsumi,
Protein, Bioconjugate Chem. 2011, 22, 315-
Y., Hatanaka, Y. [3-(Trifluoromethyl)-3H-
diazirin-3-yl]coumarin
6. Masuda, S., Tomohiro, T., Hatanaka Y. Rapidly
generating photocross-linker with masked
photoactivatable ATP probes for specific
fluorogenic beacon. Chem. Commun. 2013,
labeling of tropomyosin within
49, 11551-11553.
actomyosin protein complex, Bioorg. Med.
2. Aswad, M., Chiba, J., Tomohiro, T., Hatanaka,
Chem. Lett. 2011, 21, 2252-2254.
Y. Coupling reaction of thioamides with
7. Kashiwayama, Y., Tomohiro, T., Narita, K.,
sulfonyl azides: an efficient catalyst-free
Suzumura, M., Glumoff, T., Hiltunen, J. K., Van
click-type ligation under mild conditions.
Veldhoven, P. P., Hatanaka, Y., Imanaka, T.
Chem. Commun. 2013, 49, 10242-10244.
Identification of a substrate-binding site in a
3. Tomohiro, T., Inoguchi, H., Masuda, S.,
Hatanaka, Y. Affinity-based fluorogenic
photoaffinity labeling with a novel palmitoyl
labeling of ATP-binding proteins with
derivative. J. Biol. Chem. 2010, 285, 26315-
sequential photoactivatable cross-linkers.
Bioorg. Med. Chem. Lett. 2013, 23, 5605-
8. Review: Tomohiro, T., Hatanaka, Y. Diazirine-
based multifunctional photo-probes for
4. Morimoto, S., Tomohiro, T., Maruyama, N.,
affinity-based elucidation of protein-ligand
Hatanaka, Y. Photoaffinity casting of a
interaction. Heterocycles 2014, 89, 2697-
coumarin flag for rapid identification of
Patent:
8 patents and 4 patent-pending
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
CV OF SPEAKER
Wangsa Tirta Ismaya, PhD Education:
Universitas Padjadjaran 1993-1998 Chemistry / Biochemistry · Bachelor of Sciences
Indonesia · Bandung
University of Groningen 2002-2011 Biophysical Chemistry / Biochemistry · PhD
Netherlands · Groningen
Research Experience: Research Scientist
April 2013-present: Dexa Laboratories of Biomolecular Sciences · Biopharmaceuticals Technology · Recombinant Protein Therapeutics Research Group Indonesia · Cikarang
Postdoctoral Fellow Feb 2012-Jan 2013 Georgia Institute of Technology · Institute for Bioengineering and Bioscience · Bioengineering of Natural Products USA · Atlanta
April 2009-Jan 2012 Universiteit Utrecht · Department of Biochemistry and Cell Biology (DBC) · Bifunctional Protein
Netherlands · Utrecht Research Scholar
Feb 2008-Feb 2009: University of Groningen · Groningen Biomolecular Sciences and Biotechnology Institute (GBB) · Protein X-ray Crystallography Netherlands · Groningen
Teaching/Research Assistant Bandung Institute of Technology · Pusat Antar Universitas - Bioteknologi · Biokimia Indonesia · Bandung Publication: 1. Ismaya, W. T., Rozeboom, H. J., Weijn, A., Mes, J. J., Fusetti, F., Wichers, H. J., & Dijkstra, B. W.
(2011). Crystal structure of Agaricus bisporus mushroom tyrosinase: identity of the tetramer
subunits and interaction with tropolone. Biochemistry, 50(24), 5477-5486.
2. Ismaya, W. T., Rozeboom, H. J., Schurink, M., Boeriu, C. G., Wichers, H., & Dijkstra, B. W. (2011).
Crystallization and preliminary X-ray crystallographic analysis of tyrosinase from the
mushroom Agaricus bisporus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(5), 575-578.
3. Ismaya, W. T., Hasan, K., Kardi, I., Zainuri, A., Rahmawaty, R. I., Permanahadi, S., . & Soemitro, S.
(2013). Chemical modification of Saccharomycopsis fibuligera R64 α-Amylase to improve its stability against thermal, chelator, and proteolytic inactivation. Applied biochemistry and biotechnology, 170(1), 44-57.
4. Natalia, D., Vidilaseris, K., Ismaya, W. T., Puspasari, F., Prawira, I., Hasan, K., . & Soemitro, S.
(2015). Effect of introducing a disulphide bond between the A and C domains on the activity and stability of Saccharomycopsis fibuligera R64 α-amylase. Journal of biotechnology, 195, 8-14.
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
LIST OF COMMITTEES
: Pharmacy Faculty University of Muhammadiyah Surakarta
Steering Committee
: Rectorate board of UMS, Dean of Faculty of Pharmacy UMS
Organizing Committee
Chair
Dr. Azis Saifudin, Apt Email
Mobile : +62 82135670601
1. Dr. Anita Sukmawati, Apt
2. Alfian Mahardika F., Apt 3. Rahmadhani Tyas, Apt
1. Ika Trisharyanti, M.Pharm., Apt 2. Dyah Susilawati 3. Sri Rahayu
1. Dr. Erindyah Retno Wikantyasning, Apt
2. Arini Fadhilah, Apt 3. Juwita Rahmawati, Apt
4. Aan Wahyu Widodo 5. Muchamad Zein Arif 6. Lusiana Putri
7. Mahardika Putri Bestari 8. Sita Sofiana 9. Chinthia Devientasari
Registration and
1. Hidayah Karuniawati., M.Sc., Apt
2. Titis Putri I, Apt 3. Cita Hanif M, Apt
4. Avanilla Fany S., Apt 5. Fa'is Ayu Febrina 6. Umul Salamah
7. Siti Susilowati 8. Anisa Widyaratna 9. Vindhy Mulya Gustina
1. Dr. Zakky Cholisoh, Apt (plenary session) 2. Dr. Anita Sukmawati, Apt 3. Dr. Haryoto, M.Sc
4. Dr. Maryati., Apt 5. RatnaYuliani, M.Biotech,St 6. Broto Santoso, M.Sc., Apt
7. Gunawan Setiyadi., Apt 8. Suprapto, M.Sc., Apt
Scientific committee
1. Dr. Erindyah R Wikantyasning, Apt 2. Tanti Azizah, M.Sc., Apt 3. Dr. M. Da'i, Apt 4. Dr. Muhtadi, M.Si.
5. Dr. Anita Sukmawati., Apt 6. Dr. Zakky Cholisoh, Apt 7. Dr. Azis Saifudin, Apt
8. Dr. Maryati, Apt
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
9. Dra. Nurul Mutmainah, Apt
10. Arifah S Wahyuni, M.Sc., Apt 11. Dedi Hanwar, M.Si., Apt 12. Suprapto, M.Sc., Apt 13. Rima Munawaroh, M.Sc., Apt
14. RatnaYuliani, M.Biotech.St 15. Agus Purnomo, M.Biotech
1. Tanti Azizah, M.Sc., Apt 2. Normaidah, S.Farm. 3. Ana Amalia
4. Ilmi Nurul Fatkhi 5. Devy Anwar Zhelsiana 6. Martha Nadhira 7. Pratiwi Widowati
Equipment and Room
1. Suprapto, M.Sc., Apt 2. Rahmat Partono
3. Y Daru Prabowo 4. Hadi Cahyo, Apt 5. Subhan Rosyad Abidi
6. Akhmad Nafarin 7. Tifan Adji Hutama 8. Andriyanto Saputro
2. Zaenal Mustakim 3. Wisnu Adi Nugroho
4. Ongki Arbiyanto 5. Muhammad Arif Maulia Husna 6. Agung Beny Santosa
2. Awang Pribudi
3. Iwan Setiawan, Apt 4. Bagas Aji Kusuma 5. Muhammad Nur Prasetyo
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
MAP OF FACULTY OF PHARMACY
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
LIST OF TAXI AND HOTELS
LIST OF TAXI
Kosti Solo
Ph. +62-0271-856300
Ph. +62-0271-728728
Ph. +62-0271-734666
Ph. +62-0271-7004999
Ph. +62-0271-644194
Ph. +62-0271-781315
LIST OF HOTELS
Stars
Jl. Sugiyopranoto 20 Solo 57111
Telp: +62 (271) 646356
Fax: +62 (271) 644788
Jl. Gajah Mada 82, Solo 57132
Telp: +62 (271) 644144
Fax: +62 (271) 644133
Jl. Slamet Riyadi No. 6 Solo 57111 Telp: +62 (271) 666111
Fax: +62 (271) 666530
Email: reservation
com Jalan Adisucipto No 47 Solo
Telp: +62 (271) 724500
Fax: +62 (271) 724400
Jl. Dr. Soetomo Solo 57125
Telp: +62 (271) 7655888
Fax: +62 (271) 7655700
Jl. Ahmad Yani 40 Solo 57143
Telp: +62 (271) 731312
Fax: +62 (271) 738677
Jl. Slamet Riyadi 272 Solo 57131
Telp: +62 (271) 724555
Fax: +62 (271) 724666
Email: [email protected]
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
Alamat Jl. Veteran 284 Solo
Telp: +62 (271) 711011
Fax: +62 (271) 724368
Email: [email protected]
Jalan Adisucipto No 47 Solo
Telp: +62 (271) 711000
Fax: +62 (271) 736969
Jl. Duwet Raya No. 37 Pabelan
Telp: +62 (271) 765 2975
Email: [email protected]
ICB Pharma II – 31 October 2015
"Current Breakthrough in Pharmacy Materials and Analyses"
Source: http://icb-pharma.ums.ac.id/wp-content/uploads/2015/10/2nd_ICB-Pharma_ProgrammeAndAbstractBook.pdf
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© Schattauer 2011 Diagnostik und Behandlung des Gestationsdiabetes entsprechend den aktuellsten Leitlinien U. Schäfer-Graf Berliner Diabeteszentrum für Schwangere, Klinik für Gynäkologie & Geburtshilfe, St. Joseph Krankenhaus Keywords Definition Gestationsdiabetes, Insulinresistenz in der Gestational diabetes, insulin resistance in
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En abordant le sujet SE SOIGNER – LES PREMIERS SECOURS, nous entrons dans unepartie non négligeable de la survie. Accès rapide - - - - - HYGIÈNE ET SOIN – HYGIÈNE La base de l'hygiène c'est l'eau. Il est très risqué de boire de l'eau du robinet, elle peut êtreimpure à la consommation voire pire. Il existe une solution pour prévenir pas mal derisques, les pastilles Micropure Forte.