Grassroots academy gate-biotech- 2000GRASSROOTS ACADEMY
Correspondence Available Grassroots is a premier GATE-BIOTECH Coaching at New Delhi, with the Best results including Rank 49, 71, 313 in GATE 2010 and Rank 77 in GATE 2011. GRASSROOTS ACADEMY GATE-BIOTECH- 2000
GATE BIOTECHLOGY – 2000
The substrate for restriction enzyme is
(A) Single stranded RNA
(B) Partially double stranded RNA (C) Cell wall proteins (D) Double stranded DNA The G – C content of hyeterophage 13 double stranded DNA is 63%. What would you expect
the G + C content of its mRNA?
(A) About 68%
DNA helicases catalyse the reaction
(A) DNA supercoling
(B) DNA unwinding (C) Cleavage f DNA helix (D) Interconversion of DNA topoisomers In human populations 4% of the individuals are homozygous recessive to a specific RELP marker. What percentage of individuals do you expect to be heterozygous for this trait? (A) 4% Rho – dependent and rho – independent transcription termination mechanisms operate in
prokaryotes, Rho- independent termination mechanism involves
(A) Binding of the rho protein upstream of the termination element
(B) No protein factors and only RNA secondary structure and run of ‘U's
(C) Presence of UGA or UAA stop codons
(D) Binding of accessory factors at termination single
Many plasmids have amp1 marker. This implies (A) The plasmids contain genes for ampicillin biosynthesis (B) Ampicillin is required for bacterial growth after transformations (C) The plasmid contains the gene encoding β – lactamase (D) Ampicillin is essential for cell survival Choose the correct completion of the following statement. A fermenter sterilization in situ is less
efficient than continuous heat sterilization because
(A) it uses higher temperature for longer time
(B) it uses longer heating time during which heat is lost
(C) it uses larger volume and hence takes longer to cool the medium
(D) it uses steam as the heating source
Which of the following has been produced commercially from mammalian cell cultures?
(A) Plasminogen activator
(B) Antibacterial antibiotics (C) Insulin (D) Renin Mung bean nuclease could be used for (A) DNA synthesis (B) Nucleotide hydrolysis (C) Trimming single stranded regions in DNA (D) Removal of phosphate groups from the ends of the DNA 10. Phage 17 promoter containing plasmids are used for overexpression of cloned genes because
(A) Their convenient size (B) Their single stranded nature (C) Exquisite specificity of T7 RNA polymerase to phage promoters (D) T7 infects E. coli and lysogenizes the cell Grassroots,2274, Hudson line, Kingsway Camp, Near Delhi Univ, New Delhi, 9891602060,
GRASSROOTS ACADEMY GATE-BIOTECH- 2000
11. In eukaryotes the ribosomal RNA genes are transcribed by
(A) Reverse transcriptase (B) RNA dependent RNA polymerase (C) RNA polymerase I (D) RNA polymerase III 12. Yeast artificial chromosome (YAC) is used for
(A) Cloning large segments of DNA (B) Cloning only genomic sequences (C) Cloning of only cDNA sequences (D) All DNA except plant DNA sequences 13. The rerm protein splicing refers to
(A) Removal of intervening sequences between the genes (B) Splicing out of introns from RNA (C) Removal of intervening protein sequences from the translated protein (D) Joining (splicing) of two different gene products to generate a novel protein 14. Which one of the following is not a requirement for a PCR reaction?
(A) DNA template (B) Taq polymerase 15. In secondary metabolism two distinct phases- trophophase and idiophase refer respectively to
(A) Growth and production phase (B) Early and late phase (C) Primary and secondary metabolism (D) Lag phase and log phase 16. Which of the following eukaryotic organism has been proven to be of great industrial
(A) Pencillium chrysogenum
(B) Saccharomyces cerevisiae
(C) Bacillus subtilis
(D) Streptomyces grisents
17. Plants breeders have an advantage over animal breeders in reproducing a desired type
offspring because the plant breeders can employ
(A) Gene mutations
(C) Clonal propagation
18. Which one of the following options related to the following statement is incorrect? In comparison
to physical / chemical methods of clean up, bioremediation methods
(A) Use relatively simple techniques
(B) Generally end up with hazardous waste material
(C) Are relatively slow
(D) Are unobtrusive and non-disruptive
19. Abzymes are
(A) Enzymes that are highly specific like antibodies (B) Antibodies that have catalytic activities (C) Also referred to as zymogens (D) Enzymes which hydrolyse antibodies 20. cDNA made from the mRNA of an organism was used to make a cDNA library in a vector that
allows the expression as a fusion with a reporter tag. What percentage of the cDNA clones is likely to give rise to correct gene products? Grassroots,2274, Hudson line, Kingsway Camp, Near Delhi Univ, New Delhi, 9891602060,
GRASSROOTS ACADEMY GATE-BIOTECH- 2000
21. Commonly used reporter gene in plant expression vectors is
(A) Ti gene of Agrobacterium tumifaciens (B) GUS gene (C) β – lactamase gene (D) α – amylase gene 22. Which one of the following is not a protease?
(C) Chrymotrypsin (D) Peptidyl tRNA hydrolase 23. The Pasteur Effect is
(A) Inhibition of glucose utilization and lactate accumulation in glycolysis (B) Sterilization of milk (C) Vaccine production (D) Heat treatment of bacteria 24. Detection of which hormone is the commonly used test for pregnancy in humans?
(C) Charionic gonadotropin 25. PKU is one of the best known hereditary disorders in amino acid metabolism. The defects is
attributed to a lesion in one of the following enzymatic activities (A) Phenylalanine ammonia lyase (B) Phenylalanine hydroxylase (C) Tryosine hydroxylase (D) Phenylalanine transaminase 26. (a) Match the column A with those in column B
(A) Chemical sequencing of DNA (B) DNA blotting (2) Temin, Baltimore & Dulbecco (C) Monoclonal antibodies (D) Reverse transcription (4) Maxam and Gilbert (E) Protein sequencing (5) Kohler and Milstein (F) Polymerase chain reaction
(b) If all the steps in a PCR reaction were to work at 100% efficiency, how many micrograms of
1 Kb product will be generated from 1 p mole of DNA template after 10 cycles (1 bp= 660 Da)
27. Write whether the following statements are true or false
(A) Three important characteristics in performance of biosensors are selectivity, sensitivity and (B) Activated sludge process is one of the most common anaerobic sewage treatment methods (C) A pressure eycie is a type of air lift fermentor (D) Monoclonal antibodies are used extensively in diagnosis of haematopoietic cancers 28. (a) Explain in one sentence why you can not have monoclonal antibodies which can react with
(b) What are bispecific monoclonal antibodies?
(c) How bispecific monoclonal antibodies can be generated.
(Give two methods) 29. (a) What is somatic embryogenesis?
(b) What is the difference between direct and indirect somatic embryogenesis?
Grassroots,2274, Hudson line, Kingsway Camp, Near Delhi Univ, New Delhi, 9891602060,
GRASSROOTS ACADEMY GATE-BIOTECH- 2000
(c) State two methods for direct DNA transfer into plant cells
30. The double stranded DNA molecule of a virus was found by electron microsopy to have a length
How many nucleotide pairs are there in one molecule? How many complete turns of the two polynucleotide chains are present in such a double helix? 31 A protein PZ is present in genetically engineered bacteria at 5% of the total protein (0.1 pico
gram) per cell 1 ml of log phase culture contains 2 X 108 cells while stationary phase culture contains 1 x 10 4 cells. The molecular weight of the protein is 30,000 Da and the Avogadro number is 6.02 x 1023 molecules / mole. What is the number of molecules of PZ per cell? Calculate the amount of protein in milligrams in one liter each of log phase and stationary phase cultures. Grassroots,2274, Hudson line, Kingsway Camp, Near Delhi Univ, New Delhi, 9891602060,
SMU Medical Journal (Volume – 1, No. – 1, January 2014) Extra Esophageal Reflux Disease : An ENT Challenge Soumyajit Das1*, Suvamoy Chakraborty2 and Pronoti Sinha3 1Assistant Professor, Department of ENT and Head and Neck Surgery, SMIMS, Gangtok 2Professor and Head,Department of ENT and Head And Neck SurgerySMIMS, Gangtok.
Int.J.Curr.Microbiol.App.Sci (2014) 3(9) 573-581 ISSN: 2319-7706 Volume 3 Number 9 (2014) pp. 573-581 Original Research Article Studies on the comparison of phytochemical constituents and antimicrobial activity of Curcuma longa varieties S.Shanmugam* and P.Bhavani