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Product Data - DIO SERIES DIETS
Report Repeat Revise The "Original" High-Fat Diets for Diet Induced ObesityFormulated by E. A. Ulman, Ph.D., Research Diets, Inc., 8/26/98 and 3/11/99. Research Diets, Inc. formulated the "original" high-fat diet for diet induced obesity (DIO) studies in 1996. Today, our high-fat diets are the research standard for DIO mice and rats worldwide. Cellulose, BW200 Mineral Mix S10026 DiCalcium Phosphate Calcium Carbonate DIO Low-Fat Control Diets Potassium Citrate, 1 H2O Matched, Purified Ingredient Diet Vitamin Mix V10001 We recommend that you use a matched, purified ingredient diet Choline Bitartrate and not a grain-based 'chow' diet. There are many, many differences between purified diets and chow diets and these variables make it FD&C Red Dye #40 difficult to interpret your data from a study in which one group FD&C Blue Dye #1 was fed a purified ingredient high-fat and the other a low-fat chow diet. Differences between your groups could be due to the level of fat, but could also be due to differences in fiber type and level, source of carbohydrate, and the presence or absence of plant *Typical analysis of cholesterol in chemicals (such as phytoestrogens), just to name a few.
lard = 72 mg per 100 gram.
See next page for low-fat control formulas. Cholesterol (mg)/4057 kcal = 167.8 Cholesterol (mg)/kg = 195.5 Cholesterol (mg)/4057 kcal = 216.4 Cholesterol (mg)/kg = 279.6 Research Diets, Inc.
New Brunswick, NJ 08901 USA Tel: 732.247.2390 Fax: 732.247.2340 Where NutriPhenomics Begins 2011 Research Diets, Inc. All rights reserved. DIO-3000-2-13

Product Data - DIO SERIES DIETS
Low-Fat Control FormulasThere are many options for low-fat control diets. Here are a few examples: D12450BContains 35% sucrose by energy, unlike D12451 and D12492. Sucrose is made up of glucose and fructose and it has been shown that diets very high in sucrose or fructose ( 60-70% by energy) can induce hypertriglyceridemia, insulin resistance and fatty liver.
Match Sucrose CaloriesKeep the amount of sucrose (as a percent of calories) constant across low and high-fat diets. D12450H matches the sucrose calories in D12451. D12450J matches the sucrose calories in D12492.
These diets are formulated in such a way that when animals in the low and high-fat groups consume the same number of calories, they will also consume the same amount of sucrose.
Replace Sucrose with Corn StarchD12450K contains no sucrose but only corn starch and maltodextrin as the sources of carbohydrate. Maltodextrin is a partially hydrolyzed *Typical analysis of cholesterol in form of corn starch that allows us to produce a quality pellet in a high lard = 72 mg per 100 gram.
corn starch diet.
D12450B -
Cholesterol (mg)/4057 kcal = 54.4 There are more possible low-fat diet formulas. Please contact one of Cholesterol (mg)/kg = 51.6 our scientists and we can help you decide if one of the above diets or perhaps a different, custom diet is better for your research.
D12451 Match
D12492 Match
35% Sucrose
17% Sucrose
7% Sucrose
No Sucrose
Product #
Mineral Mix S10026 DiCalcium Phosphate Calcium Carbonate Potassium Citrate, 1 H2O Vitamin Mix V10001 Choline Bitartrate FD&C Yellow Dye #5 D12451, D12492 The "Original High-Fat Diets"
D12451, D12492 The "Original High-Fat Diets"
These are just a sample of the hundreds of citations comprising the body of scientific work built These are just a sample of the hundreds of citations comprising the body of scientific work built around these OpenSource diet formulas. around these OpenSource diet formulas. 1. Anderson EJ, Lustig ME, Boyle KE, et al. Mitochondrial H 2 O 2 emission and 1. Ahmadie R, Santiago J-jon, Walker J, et al. A High-Lipid Diet Potentiates Left cellular redox state link excess fat intake to insulin resistance in both rodents and Ventricular Dysfunction in Nitric Oxide Synthase 3-Deficient Mice after Chronic humans. J Clin Invest. 2009;119(3):573–581. Pressure Overload. Journal of Nutrition. 2010;140(June):1438-1444.
2. Burgmaier M, Sen S, Philip F, et al. Metabolic adaptation follows contractile 2. Atsui YM, Irasawa YH, Ugiura TS, et al. Metformin Reduces Body Weight Gain dysfunction in the heart of obese zucker rats fed a high-fat "Western" diet. Obesity and Improves Glucose Intolerance in High-Fat Diet-Fed C57BL / 6J Mice. Biol. (Silver Spring, Md.). 2010;18(10):1895-901. Pharm. Bull. 2010;33(June):963-970.
3. Chan C, Leo DD, Joseph J, McQuaid T. Increased uncoupling protein-2 levels in β 3. Bradford EM, Miller ML, Prasad V, et al. CLIC5 mutant mice are resistant to -cells are associated with impaired glucose-stimulated insulin secretion. Diabetes. diet-induced obesity and exhibit gastric hemorrhaging and increased susceptibility to 2001;50(June):1302-1310. 4. Chelikani PK, Haver AC, Reidelberger RD. Intermittent intraperitoneal infusion torpor. American Journal of Physiology-Regulatory, Integrative and Comparative of peptide YY(3-36) reduces daily food intake and adiposity in obese rats. Physiology. 2010;298(6):R1531. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology.
4. Chung S, Wong T, Nagasaki H, Civelli O. Acute Homeostatic Responses to 2007;293(1):R39-46. Increased Fat Consumption in MCH1R Knockout Mice. Journal of Molecular 5. Dorfman SE, Laurent D, Gounarides JS, et al. Metabolic implications of dietary Neuroscience. 2010;Online(April 22).
trans-fatty acids. Obesity (Silver Spring, Md.). 2009;17(6):1200-7. 5. Church C, Moir L, Mcmurray F, et al. Overexpression of Fto leads to increased food 6. Erickson SD, Banner B, Berthel S, et al. Potent, selective MCH-1 receptor intake and results in obesity. Nature Genetics. 2010;42(November):1086–1092.
antagonists. Bioorganic & Medicinal Chemistry Letters. 2008;18(4):1402-6. 6. Cooksey RC, Jones D, Gabrielsen S, et al. Dietary iron restriction or iron chelation 7. Esler WP, Rudolph J, Claus TH, et al. Small-Molecule Ghrelin Receptor Antagonists protects from diabetes and loss of $ beta$-cell function in the obese (ob/ob lep-/-) Improve Glucose Tolerance , Suppress Appetite , and Promote. Receptor. mouse. American Journal of Physiology-Endocrinology And Metabolism. 2010;298(6):E1236. 8. Fong TM, Guan X-M, Marsh DJ, et al. Antiobesity efficacy of a novel cannabinoid-1 7. Coppey L, Davidson E, Lu B, Gerard C, Yorek M. Neuropharmacology Vasopeptidase receptor inverse agonist, N-[(1S,2S)-3-(4-chlorophenyl)-2-(3-cyanophenyl)-1- inhibitor ilepatril ( AVE7688 ) prevents obesity- and diabetes-induced neuropathy in C57Bl / 6J mice. Neuropharmacology. 2011;60(February-March):259-266. (MK-0364), in rodents. The Journal of Pharmacology and Experimental Therapeutics.
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size after rosiglitazone treatment in high- and low-fat-fed rats. Obesity (Silver 12. Faleck D, Ali K, Roat R, et al. Adipose differentiation-related protein regulates lipids Spring, Md.). 2007;15(11):2653-63. and insulin in pancreatic islets. American Journal of Physiology-Endocrinology And 13. Kishino E, Ito T, Fujita K, Kiuchi Y. A Mixture of the Salacia reticulata (Kotala Metabolism. 2010;299(2):E249. himbutu) Aqueous Extract and Cyclodextrin Reduces the Accumulation of Visceral 13. Gallou-Kabani C, Gabory A, Al. E. Sex- and Diet-Specific Changes of Imprinted Fat Mass in Mice and Rats with High-Fat Diet–Induced Obesity. Gene Expression and DNA Methylation in Mouse Placenta under a High-Fat Diet. The Journal of Nutrition. 2006;136(2):433.
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21. Omagari K, Kato S, Tsuneyama K, et al. Effects of a long-term high-fat diet and 21. Mu J, Woods J, Zhou Y-ping, et al. Chronic Inhibition of Dipeptidyl Peptidase-4 switching from a high-fat to low-fat, standard diet on hepatic fat accumulation in With a Sitagliptin Analog Preserves Pancreatic beta -Cell Mass and Function in a Sprague-Dawley rats. Digestive Diseases and Sciences. 2008;53(12):3206-12. Rodent Model of Type 2 Diabetes. Diabetes. 2006;55(June):1695-1704. 22. Picó C, Oliver P, Sánchez J, et al. The intake of physiological doses of leptin during 22. Rabot S, Membrez M, Bruneau A, et al. Germ-free C57BL/6J mice are resistant to lactation in rats prevents obesity in later life. International Journal of Obesity (2005). high-fat-diet-induced insulin resistance and have altered cholesterol metabolism. 2007;31(8):1199-209. The FASEB Journal. 2010;24(12):4948-4959.
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high-fat diet increases susceptibility to diet-induced obesity in rat offspring. Diabetes.
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